Abstract
Biocontrol agents have been shown to be an effective alternative to chemical fungicides. Nevertheless, the main difficulties in the usage of these biocontrol agents are the development of suitable formulations and delivery systems to be applied in commercial conditions. This work aimed to evaluate the storage stability, physicochemical and rehydration properties, and biocontrol efficacy against Penicillium italicum on Persian lime of Meyerozyma guilliermondii microencapsulated in soy protein isolate-sodium alginate by spray-drying process. The bioformulation viability was 10.2 Log CFU g-1; however, its viability decreased after 120 d storage to 6.8 (4°C) and 5.5 Log CFU g-1 (25°C). Desirable physicochemical values of the bioformulation were obtained. Low humidity (5.7 %), Aw (0.29) and hygroscopicity (8.2 %), and high dispersibility values (94 %) were observed. The M. guilliermondii bioformulation effectively reduced the incidence (50 %) and severity (46 %) of blue mold on Persian lime, as well as the fresh cells of this yeast. FTIR, DSC, and TGA analysis revealed interactions of wall materials. The M. guilliermondii bioformulation can be used as an effective alternative to chemical fungicides for the management of postharvest diseases in citrus fruit.
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